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KMID : 0380620170490040453
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Korean Journal of Food Science and Technology
2017 Volume.49 No. 4 p.453 ~ p.458
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A comparative study of the immuno-modulatory activities of ethanol extracts and crude polysaccharide fractions from Annona muricata L.
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Kim Yi-Eun
Lee Joeng-Hee
Sung Nak-Yun
Ahn Dong-Hyun
Byun Eui-Hong
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Abstract
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This study compared the immuno-modulatory effects of ethanol extracts (A. muricata L. ethanol extracts, ALE) and crude polysaccharide fraction (A. muricata L. crude polysaccharide fraction, ALP) from Annona muricata L. in macrophages. Immuno-modulatory activity was determined by assessing cell viability, nitric oxide (NO) production, inducible NO synthase (iNOS) expression and cytokine production in RAW 264.7 a macrophage cell line. Both ALE and ALP treatment did not affect cytotoxicity, and ALP treatment significantly increased NO production. Additionally, cytokine production [tumor necrosis factor (TNF-α; 2909.04±4.1 pg/mL), interleukin (IL)-6; 662.84±5.3 pg/mL, and IL-1β; 852.37±2.2 pg/mL), was highly increased in the ALP (250 μg/mL) treated group compared to the ALE (250 μg/mL) treated group (TNF-α; 1564.50±6.1 pg/mL, IL-6; 517.24±4.1 pg/mL and IL-1β; 237.23±1.8 pg/mL). Moreover, ALP treatment considerably increased the expression of mitogen-activated protein kinase (MAPKs) and nuclear factor-κB (NF-κB) in the macrophages. Therefore, ALP can induce macrophage activation through MAPK and NF-κB signaling and this can be a potential candidate for development of nutraceuticals.
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KEYWORD
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Annona muricata leaves, crude polysaccharide fraction, immuno-modulatory activity, macrophage activation, nitric oxide production, cytokine production, mitogen-activated protein kinase
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